JIS Z 2801/ISO 22196

Measurement of Antibacterial Activity on Plastics Surfaces (Plastics)

This is an internationally recognized test method for evaluating the antibacterial activity of treated plastic materials (and other non-porous surfaces of products) to inhibit or kill the growth of test microorganisms.

 

Prepare samples
Sterilization

Inoculation
Incubation

Wash / Shake out

Count
[Graphics by Dr Murray Height]

 

Standardized test organism is inoculated onto the surface of test material. The standard specifies an incubation period of 24 hours but other time periods can be accommodated. Surviving microorganisms are counted to evaluate the antimicrobial activity of the test material.


Counts are determined before and after incubation. Using a formula provided in the standard, the log of the difference between the 2 counts is determined to give a measurement of antimicrobial activity.


Organisms used in this method: Staphylococcus aureus and Escherichia coli

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